Response surface methodology was used for optimization of polygalacturonase (PG) and pectinesterase (PE) production in submerged fermentation by A.niger. A Central Composite Experimental Design was applied, consisting of 22 experiments, including eight central points. Variables studied were: fermentation time (24 to 120 h), pH (3.5 to 6.5) and initial concentration of pectin (5 to 20 g/l). Maximum PE production was 220 U/l, after 74 h of culture, in a medium containing 20 g/l of pectin (pH 6.5). The optimal conditions for PG production were pH: 4.1, 20 g/l of pectin and 94 h of fermentation with a maximum value of 1032 U/l. Under these conditions, the PE production was low (15 U/l). A liquid extract with high PG activity and low PE activity could be suitable to be used in food processing in order to reduce the production of methanol.
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机译：响应面方法被用于优化黑曲霉在深层发酵中的聚半乳糖醛酸酶（PG）和果胶酯酶（PE）的生产。应用了中央复合实验设计，包括22个实验，包括八个中心点。研究的变量为：发酵时间（24至120小时），pH（3.5至6.5）和果胶初始浓度（5至20 g / l）。培养74小时后，在含有20 g / l果胶（pH 6.5）的培养基中，最大PE产量为220 U / l。 PG生产的最佳条件是pH：4.1，果胶20 g / l和94 h发酵，最大值为1032 U / l。在这些条件下，PE产量较低（15 U / l）。具有高PG活性和低PE活性的液体提取物可能适合用于食品加工中，以减少甲醇的产生。
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